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leptomycin b  (MedChemExpress)


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    Structured Review

    MedChemExpress leptomycin b
    (A) Immunofluorescence staining of HDAC1 (red) and viral ICP4 (green) in HSV-1-infected HeLa cells (MOI = 1) ± <t>leptomycin</t> <t>B</t> (LMB; 10 nM; 24 h). Scale bar: 10 μm. (B) CHX chase assay measuring HDAC1/2 degradation kinetics in HSV-1-infected HeLa cells (MOI = 1) ± LMB (10 μM). (C) Ubiquitination assay of HDAC1 in HSV-1-infected HeLa cells (MOI = 1) ± LMB (10 μM; 24 h). (D) Immunofluorescence analysis of MDM2 (red) and ICP4 (green) in HSV-1-infected HeLa cells (MOI = 1) ± LMB (10 μM; 24 h). Scale bar: 10 μm. (E) Immunoblotting analysis of DDR markers (γ-H2AX) and HDAC1/2 in HSV-1-infected HeLa cells (MOI = 1) ± LMB (10 μM). (F) Viral titers in HSV-1-infected HeLa cells (MOI = 1) ± LMB (10 μM). Data are mean ± SEM; n = 3. * P < 0.05, ** P < 0.01, *** P < 0.001
    Leptomycin B, supplied by MedChemExpress, used in various techniques. Bioz Stars score: 94/100, based on 27 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/leptomycin b/product/MedChemExpress
    Average 94 stars, based on 27 article reviews
    leptomycin b - by Bioz Stars, 2026-02
    94/100 stars

    Images

    1) Product Images from "The targeted cytosolic degradation of class I histone deacetylases is essential for efficient alphaherpesvirus replication"

    Article Title: The targeted cytosolic degradation of class I histone deacetylases is essential for efficient alphaherpesvirus replication

    Journal: bioRxiv

    doi: 10.64898/2026.01.02.697337

    (A) Immunofluorescence staining of HDAC1 (red) and viral ICP4 (green) in HSV-1-infected HeLa cells (MOI = 1) ± leptomycin B (LMB; 10 nM; 24 h). Scale bar: 10 μm. (B) CHX chase assay measuring HDAC1/2 degradation kinetics in HSV-1-infected HeLa cells (MOI = 1) ± LMB (10 μM). (C) Ubiquitination assay of HDAC1 in HSV-1-infected HeLa cells (MOI = 1) ± LMB (10 μM; 24 h). (D) Immunofluorescence analysis of MDM2 (red) and ICP4 (green) in HSV-1-infected HeLa cells (MOI = 1) ± LMB (10 μM; 24 h). Scale bar: 10 μm. (E) Immunoblotting analysis of DDR markers (γ-H2AX) and HDAC1/2 in HSV-1-infected HeLa cells (MOI = 1) ± LMB (10 μM). (F) Viral titers in HSV-1-infected HeLa cells (MOI = 1) ± LMB (10 μM). Data are mean ± SEM; n = 3. * P < 0.05, ** P < 0.01, *** P < 0.001
    Figure Legend Snippet: (A) Immunofluorescence staining of HDAC1 (red) and viral ICP4 (green) in HSV-1-infected HeLa cells (MOI = 1) ± leptomycin B (LMB; 10 nM; 24 h). Scale bar: 10 μm. (B) CHX chase assay measuring HDAC1/2 degradation kinetics in HSV-1-infected HeLa cells (MOI = 1) ± LMB (10 μM). (C) Ubiquitination assay of HDAC1 in HSV-1-infected HeLa cells (MOI = 1) ± LMB (10 μM; 24 h). (D) Immunofluorescence analysis of MDM2 (red) and ICP4 (green) in HSV-1-infected HeLa cells (MOI = 1) ± LMB (10 μM; 24 h). Scale bar: 10 μm. (E) Immunoblotting analysis of DDR markers (γ-H2AX) and HDAC1/2 in HSV-1-infected HeLa cells (MOI = 1) ± LMB (10 μM). (F) Viral titers in HSV-1-infected HeLa cells (MOI = 1) ± LMB (10 μM). Data are mean ± SEM; n = 3. * P < 0.05, ** P < 0.01, *** P < 0.001

    Techniques Used: Immunofluorescence, Staining, Infection, Ubiquitin Proteomics, Western Blot



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    (A) Immunofluorescence staining of HDAC1 (red) and viral ICP4 (green) in HSV-1-infected HeLa cells (MOI = 1) ± <t>leptomycin</t> <t>B</t> (LMB; 10 nM; 24 h). Scale bar: 10 μm. (B) CHX chase assay measuring HDAC1/2 degradation kinetics in HSV-1-infected HeLa cells (MOI = 1) ± LMB (10 μM). (C) Ubiquitination assay of HDAC1 in HSV-1-infected HeLa cells (MOI = 1) ± LMB (10 μM; 24 h). (D) Immunofluorescence analysis of MDM2 (red) and ICP4 (green) in HSV-1-infected HeLa cells (MOI = 1) ± LMB (10 μM; 24 h). Scale bar: 10 μm. (E) Immunoblotting analysis of DDR markers (γ-H2AX) and HDAC1/2 in HSV-1-infected HeLa cells (MOI = 1) ± LMB (10 μM). (F) Viral titers in HSV-1-infected HeLa cells (MOI = 1) ± LMB (10 μM). Data are mean ± SEM; n = 3. * P < 0.05, ** P < 0.01, *** P < 0.001
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    (A) Immunofluorescence staining of HDAC1 (red) and viral ICP4 (green) in HSV-1-infected HeLa cells (MOI = 1) ± <t>leptomycin</t> <t>B</t> (LMB; 10 nM; 24 h). Scale bar: 10 μm. (B) CHX chase assay measuring HDAC1/2 degradation kinetics in HSV-1-infected HeLa cells (MOI = 1) ± LMB (10 μM). (C) Ubiquitination assay of HDAC1 in HSV-1-infected HeLa cells (MOI = 1) ± LMB (10 μM; 24 h). (D) Immunofluorescence analysis of MDM2 (red) and ICP4 (green) in HSV-1-infected HeLa cells (MOI = 1) ± LMB (10 μM; 24 h). Scale bar: 10 μm. (E) Immunoblotting analysis of DDR markers (γ-H2AX) and HDAC1/2 in HSV-1-infected HeLa cells (MOI = 1) ± LMB (10 μM). (F) Viral titers in HSV-1-infected HeLa cells (MOI = 1) ± LMB (10 μM). Data are mean ± SEM; n = 3. * P < 0.05, ** P < 0.01, *** P < 0.001
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    (A) Immunofluorescence staining of HDAC1 (red) and viral ICP4 (green) in HSV-1-infected HeLa cells (MOI = 1) ± <t>leptomycin</t> <t>B</t> (LMB; 10 nM; 24 h). Scale bar: 10 μm. (B) CHX chase assay measuring HDAC1/2 degradation kinetics in HSV-1-infected HeLa cells (MOI = 1) ± LMB (10 μM). (C) Ubiquitination assay of HDAC1 in HSV-1-infected HeLa cells (MOI = 1) ± LMB (10 μM; 24 h). (D) Immunofluorescence analysis of MDM2 (red) and ICP4 (green) in HSV-1-infected HeLa cells (MOI = 1) ± LMB (10 μM; 24 h). Scale bar: 10 μm. (E) Immunoblotting analysis of DDR markers (γ-H2AX) and HDAC1/2 in HSV-1-infected HeLa cells (MOI = 1) ± LMB (10 μM). (F) Viral titers in HSV-1-infected HeLa cells (MOI = 1) ± LMB (10 μM). Data are mean ± SEM; n = 3. * P < 0.05, ** P < 0.01, *** P < 0.001
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    (A) Immunofluorescence staining of HDAC1 (red) and viral ICP4 (green) in HSV-1-infected HeLa cells (MOI = 1) ± <t>leptomycin</t> <t>B</t> (LMB; 10 nM; 24 h). Scale bar: 10 μm. (B) CHX chase assay measuring HDAC1/2 degradation kinetics in HSV-1-infected HeLa cells (MOI = 1) ± LMB (10 μM). (C) Ubiquitination assay of HDAC1 in HSV-1-infected HeLa cells (MOI = 1) ± LMB (10 μM; 24 h). (D) Immunofluorescence analysis of MDM2 (red) and ICP4 (green) in HSV-1-infected HeLa cells (MOI = 1) ± LMB (10 μM; 24 h). Scale bar: 10 μm. (E) Immunoblotting analysis of DDR markers (γ-H2AX) and HDAC1/2 in HSV-1-infected HeLa cells (MOI = 1) ± LMB (10 μM). (F) Viral titers in HSV-1-infected HeLa cells (MOI = 1) ± LMB (10 μM). Data are mean ± SEM; n = 3. * P < 0.05, ** P < 0.01, *** P < 0.001
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    (A) Immunofluorescence staining of HDAC1 (red) and viral ICP4 (green) in HSV-1-infected HeLa cells (MOI = 1) ± <t>leptomycin</t> <t>B</t> (LMB; 10 nM; 24 h). Scale bar: 10 μm. (B) CHX chase assay measuring HDAC1/2 degradation kinetics in HSV-1-infected HeLa cells (MOI = 1) ± LMB (10 μM). (C) Ubiquitination assay of HDAC1 in HSV-1-infected HeLa cells (MOI = 1) ± LMB (10 μM; 24 h). (D) Immunofluorescence analysis of MDM2 (red) and ICP4 (green) in HSV-1-infected HeLa cells (MOI = 1) ± LMB (10 μM; 24 h). Scale bar: 10 μm. (E) Immunoblotting analysis of DDR markers (γ-H2AX) and HDAC1/2 in HSV-1-infected HeLa cells (MOI = 1) ± LMB (10 μM). (F) Viral titers in HSV-1-infected HeLa cells (MOI = 1) ± LMB (10 μM). Data are mean ± SEM; n = 3. * P < 0.05, ** P < 0.01, *** P < 0.001
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    Image Search Results


    (A) Immunofluorescence staining of HDAC1 (red) and viral ICP4 (green) in HSV-1-infected HeLa cells (MOI = 1) ± leptomycin B (LMB; 10 nM; 24 h). Scale bar: 10 μm. (B) CHX chase assay measuring HDAC1/2 degradation kinetics in HSV-1-infected HeLa cells (MOI = 1) ± LMB (10 μM). (C) Ubiquitination assay of HDAC1 in HSV-1-infected HeLa cells (MOI = 1) ± LMB (10 μM; 24 h). (D) Immunofluorescence analysis of MDM2 (red) and ICP4 (green) in HSV-1-infected HeLa cells (MOI = 1) ± LMB (10 μM; 24 h). Scale bar: 10 μm. (E) Immunoblotting analysis of DDR markers (γ-H2AX) and HDAC1/2 in HSV-1-infected HeLa cells (MOI = 1) ± LMB (10 μM). (F) Viral titers in HSV-1-infected HeLa cells (MOI = 1) ± LMB (10 μM). Data are mean ± SEM; n = 3. * P < 0.05, ** P < 0.01, *** P < 0.001

    Journal: bioRxiv

    Article Title: The targeted cytosolic degradation of class I histone deacetylases is essential for efficient alphaherpesvirus replication

    doi: 10.64898/2026.01.02.697337

    Figure Lengend Snippet: (A) Immunofluorescence staining of HDAC1 (red) and viral ICP4 (green) in HSV-1-infected HeLa cells (MOI = 1) ± leptomycin B (LMB; 10 nM; 24 h). Scale bar: 10 μm. (B) CHX chase assay measuring HDAC1/2 degradation kinetics in HSV-1-infected HeLa cells (MOI = 1) ± LMB (10 μM). (C) Ubiquitination assay of HDAC1 in HSV-1-infected HeLa cells (MOI = 1) ± LMB (10 μM; 24 h). (D) Immunofluorescence analysis of MDM2 (red) and ICP4 (green) in HSV-1-infected HeLa cells (MOI = 1) ± LMB (10 μM; 24 h). Scale bar: 10 μm. (E) Immunoblotting analysis of DDR markers (γ-H2AX) and HDAC1/2 in HSV-1-infected HeLa cells (MOI = 1) ± LMB (10 μM). (F) Viral titers in HSV-1-infected HeLa cells (MOI = 1) ± LMB (10 μM). Data are mean ± SEM; n = 3. * P < 0.05, ** P < 0.01, *** P < 0.001

    Article Snippet: Reagents were sourced as follows: Leptomycin B (HY-16909), MG-132 (HY-13259), 3-MA (HY-19312), cycloheximide (HY-12320), and chloroquine (HY-17589A) were purchased from MedChemExpress; berzosertib (S7102) was purchased from Selleck; DMSO (W387520) was purchased from Sigma-Aldrich.

    Techniques: Immunofluorescence, Staining, Infection, Ubiquitin Proteomics, Western Blot